糖蛋白130和c-kit同时激活对脐血CD34+细胞的扩增作用

目的 :探讨同时激活糖蛋白 1 30 ( gp1 30 )和 c- kit信号系统 ,对脐血长期培养启动细胞 ( LTC-IC)和混合造血细胞集落形成单位 ( CFU- Mix)的扩增作用。方法 :利用免疫磁珠法分离纯化脐血 CD 3 4细胞 ,在体外液体培养体系中 ,经可溶性白细胞介素 6受体 ( s IL- 6R)、白细胞介素 6( IL- 6)、干细胞因子 ( SCF)和白细胞介素 3( IL- 3)的不同因子及组合扩增 3w,分别用甲基纤维素法和有限稀释法测定 CFU- Mix和 LTC- IC的扩增效率。结果 :s IL- 6、IL- 6和 SCF单独都不能使 CFU- Mix得到扩增 ,而三者联合可使 CFU- Mix数目显著扩增 ,且于培养 2 w时扩增效能最佳 ,此时 CFU- Mix扩增到 2 2 .5倍 ,LTC- IC扩增到 3.5倍 ,作为对照的 SCF IL- 6 IL- 3组则仅分别扩增到 3.0和 1 .9倍。形态学分析发现 :IL- 6、s IL- 6R和 SCF协同作用下 ,产生的集落体积较大 ,并可观察到各系造血细胞集落及不同发展阶段的细胞。结论 :由 IL- 6/s IL- 6R复合物和 SCF联合激活 gp1 30和 c- kit信号系统可刺激造血干 /祖细胞发生明显扩增 ,体外扩散时间以 1～ 2周为宜。     

草酸钙沉淀作为离子色谱尿草酸测定的尿样预处理法

为了消除离子色谱法测定尿液草酸浓度时部分尿样中存在的杂质峰对草酸测定的干扰,先在尿液中加入氯化钙而发生草酸钙沉淀,再用盐酸溶解该沉淀即可达到清除杂质峰的目的。结果显示草酸钙沉淀尿样预处理法能消除杂质峰对草酸峰的干扰,预处理过程中草酸丢失极少,草酸回收率达96%～98%。结果提示该预处理法可用于存在杂质峰尿样的尿草酸的测定。     

快速酶免疫法与粪检法检测华支睾吸虫病的比较分析

目前,华支睾吸虫的诊断主要依靠快速酶免疫诊断试剂盒和粪检,为对这二种方法进行比较,选３５６名学生,同时进行血清学抗体和粪便虫卵的检测,抗体阳性率８．７１％（３１／３５６）,虫卵阳性率８．１５％（２９／３５６）,二者无显著性差异,其中二法均阳性２６例,阳性符合率８９．６６％,二法均阴性３２２例,阴性符合率９８．４７％,总符合率９７．７５％,表明快速酶免疫法与粪检法作为诊断华支睾吸虫病的二种不同的检测     

树脂法生产龟鹿补肾液的工艺研究

对龟鹿补肾液的生产工艺进行了改进研究，由原来的酒沉法改为树脂法。对两种工艺试制的口服液成品，通过淫羊藿甙、蛋白质、多糖等成分的含量比较，表明树脂法工艺试制口服液成品的各项指标有明显提高，并保存了原有较佳的外观质量。     

Quantitation of low level unconjugated polysaccharide in tetanus toxoid-conjugate vaccine by HPAEC/PAD following rapid separation by deoxycholate/HCl

A simple and rapid acid precipitation method has been applied successfully for separating free capsular polysaccharide of Haemophilus influenzae type b (polyribosyl ribitol phosphate, PRP) from PRP–tetanus toxoid conjugate (PRP-T) in a final dosage amount of low-level materials. The unconjugated PRP was found to stay in the supernatant without precipitation, while conjugated PRP-T was fully precipitated. High performance anion exchange chromatography with pulsed amperometric detection (HPAEC-PAD) has been applied for analysis of the PRP content in the supernatant after the separation. This method requires minimum sample handling and is specific, sensitive and reproducible making it suitable for release and stability testing of PRP-T in final containers.     

In Vitro Evaluation of the Plasma and Blood Compatibility of a Parenteral Formulation for Ditekiren,a Novel Renin Inhibitor Pseudopeptide

Ditekiren (U-71038; Boc-Pro-Phe-N-MeHis-Leu-[CHOHCH₂]-Val-Ile-aminomethyl)pyridine) is a potent renin inhibitor peptide and was formulated for clinical intravenous administration in acidified dextrose. This formulation of ditekiren was evaluated in vitro with human and monkey plasma as to its potential for forming a precipitate either of drug or of plasma proteins. Analysis by centrifugation showed that no drug precipitation occurred in plasma from either species at concentrations 25 times higher than anticipated in clinical studies. Results obtained by turbidimetry indicated that formulated ditekiren did not cause aggregation of human platelets or flocculation of proteins at concentrations approaching the solubility limit of the drug in plasma. Ditekiren or vehicle also caused no detectable lysis of red cells at concentrations representing 10 times the maximum clinical level. Therefore, ditekiren solutions as formulated are judged completely compatible with blood and plasma upon clinical intravenous administration.     

Personalized Oncology in Interventional Radiology

As personalized medicine becomes more applicable to oncologic practice, image-guided biopsies will be integral for enabling predictive and pharmacodynamic molecular pathology. Interventional radiology has a key role in defining patient-specific management. Advances in diagnostic techniques, genomics, and proteomics enable a window into subcellular mechanisms driving hyperproliferation, metastatic capabilities, and tumor angiogenesis. A new era of personalized medicine has evolved whereby clinical decisions are adjusted according to a patient’s molecular profile. Several mutations and key markers already have been introduced into standard oncologic practice. A broader understanding of personalized oncology will help interventionalists play a greater role in therapy selection and discovery.     

Worldwide data sets constrain the water vapor uptake coefficient in cloud formation

Cloud droplet formation depends on the condensation of water vapor on ambient aerosols, the rate of which is strongly affected by the kinetics of water uptake as expressed by the condensation (or mass accommodation) coefficient, α_c. Estimates of α_c for droplet growth from activation of ambient particles vary considerably and represent a critical source of uncertainty in estimates of global cloud droplet distributions and the aerosol indirect forcing of climate. We present an analysis of 10 globally relevant data sets of cloud condensation nuclei to constrain the value of α_c for ambient aerosol. We find that rapid activation kinetics (α_c > 0.1) is uniformly prevalent. This finding resolves a long-standing issue in cloud physics, as the uncertainty in water vapor accommodation on droplets is considerably less than previously thought.     

茶碱分子印迹聚合物微球的合成及其性能研究

目的 制备茶碱分子印迹聚合物微球,考察模板分子与交联剂的配比、反应时间、溶剂对分子印迹聚合物微球形貌、产率以及性能的影响.方法 以茶碱为模板分子,丙烯酰胺(AM)为功能单体,乙二醇二甲基丙烯酸酯(EGDMA)为交联剂,采用沉淀聚合法合成分子印迹微球,采用静态吸附、扫描电镜(SEM)对微球进行表征.结果 当模板分子与交联剂配比为1∶16、反应时间为24 h、乙腈为溶剂时,所制得印迹聚合物微球的形貌和吸附性能较好,对茶碱与咖啡碱的选择性分离因子α为1.74.结论 分子印迹聚合物微球对茶碱分子有特异性吸附和识别能力.